Coding
TCaiiAyhjH

Part:BBa_K2471003

Designed by: Armando Palacios Chaparro   Group: iGEM17_Tec-Chihuahua   (2017-10-24)

(T7 promoter + RBS) + (aiiA) + (RBS) + (yhjH) + (RBS) + (epsE) + (T1 terminator)


This BioBrick™ contains the necessary genetic circuitry to constitutively express the aiiA, yhjH and epsE genes, that when expressed, results in the production of the three enzymes of the same name. The combined effect of these will cause the cell to be unable to communicate (quorum quenching), move (motility inhibition) and form biofilm (decrease of ci-d-GMP).


A chassis with the T7 polymerase gene is needed for the successful expression of this BioBrick™.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2434
    Illegal AgeI site found at 137
    Illegal AgeI site found at 2184
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None