Coding
TCaiiA

Part:BBa_K2471000

Designed by: Armando Palacios Chaparro   Group: iGEM17_Tec-Chihuahua   (2017-10-17)

(T7 promoter + RBS) + (aiiA) + (T1 terminator)


This BioBrick™ contains the necessary genetic circuitry to constitutively express the aiiA gene, that when expressed, results in the production of the aiiA enzyme, and thus, the quenching of quorum sensing thanks to the decrease in AHLs.


A chassis with the T7 polymerase gene is needed for the successful expression of this BioBrick™.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 137
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None