Composite

Part:BBa_K2469004

Designed by: Ahmed Ibrahim   Group: iGEM17_Toronto   (2017-10-26)


YFP-mCherry Switch

An optogenetic, light controlled switch. In the absence of light, LacILOV represses the expression of genes under the Prtc-2 promoter. Upon blue light excitation this repression is lifted, cI is expressed and able to repress the BBa_R0051 promoter. This construct demonstrates a potential use for LacILOV within the context of a light controlled genetic switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1610
    Illegal NheI site found at 1723
    Illegal NheI site found at 1746
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None