DNA

Part:BBa_K2460005

Designed by: Haiyun Liu   Group: iGEM17_Fudan_China   (2017-10-21)


φRv1 attB

This is a recombination site attB for phiRv1 integrase (BBa_K2460004). When used with phiBT1 attP (BBa_K2460006) and phiRv1 integrase, it can flip a DNA sequence flanked by attB and attP sites, as well as integrating a circular DNA with a attB/P site into a linear/circular DNA with a attP/B site. For more information about kinetic characterization and the orthogonality between the integrase and some other serine recombinase, go to phiRv1 integrase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None