Part:BBa_K2455007

YddG-TrpE construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455001)

This is a composite part consisting of the two YddG (BBa_K2455004) and TrpE (BBa_K2455001) biobricks with an RBS in between.

This biobrick has been designed in order to over-express and secrete tryptophan in E. coli.

YddG is an aromatic amino acid exporter that is responsible for the secretion of Tryptophan, Tyrosine and Phenylalanine. Previous work by Wang et al. have shown that over-expression of YddG in E.coli increases the accumulation of Tryptophan in the culture medium.

TrpE codes for the Anthranilate enzyme responsible for the first step of the conversion of Chorismate into to tryptophan. Previous work (Gu et al., 2012) have shown that over-expression of TrpE leads to increased tryptophan production in E. coli. The trpE gene carries a M293T point mutation (to avoid negative feedback inhibition) and a hexa histidine-tag attached by a short linker sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1876
  Illegal BglII site found at 1937
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 577
  Illegal AgeI site found at 639
  Illegal AgeI site found at 756
  
• 1000
  COMPATIBLE WITH RFC[1000]