Reporter

Part:BBa_K2451015

Designed by: Jared Clements   Group: iGEM17_UT-Knoxville   (2017-10-26)


Lac-RBS-xylR-Terminator-Pu-RBS-xylA-RBS-xylM-T-Lac-RBS-xylB-T

Catalytic sites of xylA, xylM, and xylB from P. putida were added in place of GFP from part BBa_K2451013. This creates a circuit for expression of monooxygenases xylA and xylM under the control of xylR (regulatory protein) and aromatic substrates. Additionally, dehydrogenase xylB is transcribed under a Lac promoter as the initial constructs (BBa_K1966000 and BBa_K1966001) were ligated together in the 2016 project. See https://parts.igem.org/Part:BBa_K2451013 for usage information.

Testing utilizing IPTG and 1 mM m-xylenes revealed the following gas chromatography data for alcohol and aldehyde conversions in M9 media. BBa_K2451015_aldehyde_graph.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4681
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5333


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