Generator

Part:BBa_K2451008

Designed by: Matthew Kubis   Group: iGEM17_UT-Knoxville   (2017-10-23)


AcrR Generator

A production circuit for the transcriptional regulator AcrR. AcrR is a transcriptional repressor in E. Coli responsible for regulating the AcrAB-TolC efflux pump. It's primary purpose is to mediate expression of non-specific RND efflux pumps; conferring susceptibility to antibiotics through repression of AcrAB-TolC. Additionally, it has been shown there is significant cross-talk between AcrR and the Mar-Sox-Rob regulon.


https://parts.igem.org/File:Benzaldehyde_Toxicity_Test.jpeg https://parts.igem.org/File:Benzaldehdye_Toxicity_Test_Controls.jpeg https://parts.igem.org/File:Ampicillin_Toxicity_Test.jpeg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 249
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None