Generator

Part:BBa_K2451006

Designed by: Matthew Kubis   Group: iGEM17_UT-Knoxville   (2017-10-23)


Rob Generator

A production circuit for inducible expression of transcriptional regulator Rob. Rob is a transcriptional regulator in E. Coli responsible for regulating >60 chromosomal genes. It's primary purpose is to mediate expression of non-specific RND efflux pumps; conferring resistance to antibiotics and organic solvents through increased expression of AcrAB-TolC. Rob is a significant actor in the Mar-Sox-Rob regulon. Contains EcoRI and PstI in the coding sequence.


https://parts.igem.org/File:Benzaldehyde_Toxicity_Test.jpeg https://parts.igem.org/File:Benzaldehdye_Toxicity_Test_Controls.jpeg https://parts.igem.org/File:Ampicillin_Toxicity_Test.jpeg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 431
    Illegal PstI site found at 722
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 431
    Illegal PstI site found at 722
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 431
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 431
    Illegal PstI site found at 722
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 431
    Illegal PstI site found at 722
    Illegal NgoMIV site found at 77
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None