Generator

Part:BBa_K2451004

Designed by: Matthew Kubis   Group: iGEM17_UT-Knoxville   (2017-10-23)


SoxS Generator

A production circuit for inducible expression of transcriptional regulator SoxS. SoxS is a transcriptional regulator in E. Coli responsible for regulating >60 chromosomal genes. It's primary purpose is to mediate expression of non-specific RND efflux pumps; conferring resistance to antibiotics and organic solvents through increased expression of AcrAB-TolC. SoxS is a significant actor in the Mar-Sox-Rob regulon.


https://parts.igem.org/File:Benzaldehyde_Toxicity_Test.jpeg https://parts.igem.org/File:Benzaldehdye_Toxicity_Test_Controls.jpeg https://parts.igem.org/File:Ampicillin_Toxicity_Test.jpeg https://parts.igem.org/File:PSoxS_Amp_Plates.jpeg https://parts.igem.org/File:PSB1C3_Amp_Plates.jpeg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None