Composite
RFC 37

Part:BBa_K245075

Designed by: Špela Miklavič   Group: iGEM09_Slovenia   (2009-10-16)

KSI-DP-EEE

Sequence codes a polypeptide consisting of ketosteroid isomerase (KSI) with additional Asp-Pro dipeptide followed fibers-forming EEE. KSI facilitates isolation of protein since it causes packing of the polypeptide in inclusion bodies. KSI can later be removed by exposing the polypeptide to acidic environment which results in cleavage of D-P peptide bond. EEE consists of three heterotrimerisation domains. Each of this domains interacts vith other two on other polypeptide chains. This interactions should result in fibes or network of interconnected fibers. Glutamic acid residues on the surface of the protein could be basis for metalisation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal NgoMIV site found at 313
    Illegal NgoMIV site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None