Composite
RFC 37

Part:BBa_K245051

Designed by: Špela Miklavič   Group: iGEM09_Slovenia   (2009-10-14)

GyrB-SG-SG-CutA1

This part is made of two domains – GyrB, a 24 kDa fragment of gyrase subunit B, and CutA1, a copper binding protein. It is one of the constructs whose assembly can be regulated. When an aminocoumarin antibiotic called coumermycin is added, GyrB domains dimerize. Because this is a combination of dimerization and trimerization domains, the resulting structure is a lattice with hexagonal pores. Another aminocoumarin antibiotic novobiocin inhibits this structure. This part is similar to GyrB-CutA1 (BBa_K245050) but has extended linker according to Standard 37.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 238
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None