Plasmid_Backbone

Part:BBa_K245008

Designed by: Nika Debeljak   Group: iGEM09_Slovenia   (2009-09-26)


BB-NIC-III-HisN

This plasmid backbone is the same as BBa_K245004 except that the restriction sites AgeI and XmaI are exchanged (which results in NgoMIV and XmaI restriction sites in prefix AgeI and BspEI in suffix). This detail enables extension of part cloned into vector with SG on C-terminal or/and PG on N-terminal.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal XbaI site found at 2244
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
    Illegal NotI site found at 70
    Illegal NotI site found at 2125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal XbaI site found at 2244
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal XbaI site found at 2244
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
    Illegal NgoMIV site found at 2250
    Illegal AgeI site found at 1
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1158


[edit]
Categories
Parameters
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