Plasmid_Backbone

Part:BBa_K245006

Designed by: Nika Debeljak   Group: iGEM09_Slovenia   (2009-09-25)


BB-NIC-II-NC

This plasmid backbone is the same as BBa_K245004 except that it doesn't contain His tag at all.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal XbaI site found at 53
    Illegal XbaI site found at 2179
    Illegal XbaI site found at 2223
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
    Illegal NotI site found at 70
    Illegal NotI site found at 2125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal XbaI site found at 53
    Illegal XbaI site found at 2179
    Illegal XbaI site found at 2223
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2119
    Illegal XbaI site found at 53
    Illegal XbaI site found at 2179
    Illegal XbaI site found at 2223
    Illegal SpeI site found at 13
    Illegal PstI site found at 77
    Illegal NgoMIV site found at 2229
    Illegal AgeI site found at 2235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1158


[edit]
Categories
Parameters
None