Part:BBa_K245005

BB-NIC-II-HisN

BB-NIC-II-HisN originates from BioBrick vector pSB1A2 but contains modified prefix and suffix according to assembly standard 37. T7 promoter, RBS and ATG are included in prefix and T7 terminator and STOP codon in suffix. His tag is also inserted between ATG and multiple cloning site in prefix. There are four new inner restriction sites added in multiple-cloning site (NgoMIV and AgeI in prefix; XmaI and BspEI in suffix) which enable extension of part cloned into BB-NIC-II-HisN with SG on N-terminal or TG on C-terminal; extension of linker and creating a friendly scar during parts fusion.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  Illegal XbaI site found at 2244
  Illegal SpeI site found at 13
  Illegal PstI site found at 77
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  Illegal SpeI site found at 13
  Illegal PstI site found at 77
  Illegal NotI site found at 70
  Illegal NotI site found at 2125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  Illegal XbaI site found at 2244
  Illegal SpeI site found at 13
  Illegal PstI site found at 77
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  Illegal XbaI site found at 2244
  Illegal SpeI site found at 13
  Illegal PstI site found at 77
  Illegal NgoMIV site found at 2250
  Illegal AgeI site found at 2256
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1158