Part:BBa_K245004

-- No Description --

BB-NIC-II-HisN originates from BioBrick vector pSB1A2 but contains modified prefix and suffix according to assembly standard 37. T7 promoter is included in prefix and T7 terminator in suffix. There are four new inner restriction sites added in multiple-cloning site (NgoMIV, AgeI-prefix; XmaI/BspEI-suffix) which enable extension of part cloned into BB-NIC-II-HisN with S/G on N-terminal or T/G on C-terminal; extension of linker and creating a friendly scar during parts fusion.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  Illegal XbaI site found at 2244
  Illegal SpeI site found at 13
  Illegal PstI site found at 77
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  Illegal SpeI site found at 13
  Illegal PstI site found at 77
  Illegal NotI site found at 70
  Illegal NotI site found at 2125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  Illegal XbaI site found at 2244
  Illegal SpeI site found at 13
  Illegal PstI site found at 77
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2119
  Illegal XbaI site found at 2244
  Illegal SpeI site found at 13
  Illegal PstI site found at 77
  Illegal NgoMIV site found at 2250
  Illegal AgeI site found at 2256
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1158