Intermediate

Part:BBa_K2449030

Designed by: Sarah JÝrgensen   Group: iGEM17_SDU-Denmark   (2017-10-25)


Shell-associated protein, carbonic anhydrase, shell-proteins, and pore protein of the carboxysome

Intermediate part containing the genes encoded by the cso operon from Halothiobacillus neapolitanus. Together, these genes encode the proteins required for the biosynthesis and assembly of a functional …Ď-carboxysome.

This microcompartment consists of a proteinaceous outer shell composed of six different shell proteins designated CsoS1ABCD and CsoS4AB. This shell encloses RuBisCo of the Calvin cycle, the shell associated protein (CsoS2), and the enzyme carbonic anhydrase (CsoS3).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2848
    Illegal AgeI site found at 851
    Illegal AgeI site found at 1802
    Illegal AgeI site found at 2483
    Illegal AgeI site found at 4959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 243


[edit]
Categories
Parameters
None