Intermediate

Part:BBa_K2449028

Designed by: Sarah Jrgensen   Group: iGEM17_SDU-Denmark   (2017-10-25)


RelB with RBS and double terminator

A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In E. coli K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1]. The part BBa_K2449029 encodes the toxin RelE.

RelB neutralize the toxic effect of RelE through interaction between the two proteins.

[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.


Sequence and Features
RelB cloned with RBS site and a double terminator.
RelB was placed under control of the pBAD/AraC promoter in composite part, BBa_K2449031.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None