Composite

Part:BBa_K2449027

Designed by: Malte Skovsager Andersen   Group: iGEM17_SDU-Denmark   (2017-10-23)


cenA-hlyA regulated by penI, with terminater

This part is coding for the cellulase endoglucanase with a hlyA-tag.The endoglucanase can in partnership with the exoglucanase break down cellulose to cellobiose. The hlyA-tag makes the endoglucanase secreteable with the alpha-hemolysin transport system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 408
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
    Illegal BamHI site found at 1470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 300
    Illegal AgeI site found at 396
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 108


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Categories
Parameters
None