Composite

Part:BBa_K2449017

Designed by: Malte Skovsager Andersen   Group: iGEM17_SDU-Denmark   (2017-09-29)


cex containg hly-tag with penI and RBS

This part is coding for the cellulase exoglucanase with a hlyA-tag.The exoglucanase can in partnership with the endoglucanase break down cellulose to cellobiose. The hlyA-tag makes the exoglucanase secreteable with the alpha-hemolysin transport system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 627
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 260
    Illegal NgoMIV site found at 633
    Illegal NgoMIV site found at 1135
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 680
    Illegal SapI.rc site found at 763


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Categories
Parameters
None