Part:BBa_K2448016

pPsiTac1

This part is a hybrid synthetic promoter composed of the -35 and the Pribnow box sequences of pTacI promoter (BBa_K864400) [1] and the consensus binding site of PsiR regulator of Rhizobiale [2].

Usage and Biology

D-Psicose inducible promoters naturally occur in nature, but they are quite long and not well characterized. In order to assess the efficiency of our psicose biosensor(s), we engineered a strong promoter, pTacI (BBa_K864400), regulated by LacI to make it psicose inducible.

pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR (BBa_K2448006). PsiR is a LacI family transcription factor interacting with psicose, this is why the regulation of its promoter is similar.

As shown by the characterization of our psicose biosensor (BBa_K2448027), pPsiTac1 is strongly regulated by PsiR thanks to the consensus sequence, and the reporter gene shows high level expression under psicose induction. pPsiTac1 can therefore be categorized as a strong psicose inducible promoter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]