Coding

Part:BBa_K2444001

Designed by: Sarah Wenyon   Group: iGEM17_Delaware   (2017-10-24)


Codes for the CvaAB transporter system

Used to interact with the Colicin V leader peptides for effective secretion of edited bacteriocins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1679
    Illegal EcoRI site found at 2346
    Illegal PstI site found at 607
    Illegal PstI site found at 820
    Illegal PstI site found at 1697
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1679
    Illegal EcoRI site found at 2346
    Illegal PstI site found at 607
    Illegal PstI site found at 820
    Illegal PstI site found at 1697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1679
    Illegal EcoRI site found at 2346
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1679
    Illegal EcoRI site found at 2346
    Illegal PstI site found at 607
    Illegal PstI site found at 820
    Illegal PstI site found at 1697
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1679
    Illegal EcoRI site found at 2346
    Illegal PstI site found at 607
    Illegal PstI site found at 820
    Illegal PstI site found at 1697
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1313
    Illegal SapI site found at 2422


[edit]
Categories
Parameters
None