Coding

Part:BBa_K2443077

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-12)


T7 RNA Polymerase

T7 RNA Polymerase is responsible for transcribing DNA into RNA and is under the regulation of the T7 promoter consensus sequence. It has been Codon optimized for use in Escherichia coli and contains a N-terminal histidine tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1670
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 1894
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None