Coding

Part:BBa_K2443070

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-10)


RF2

Release factor 2 (RF2) allows for the termination of translation through the recognition of the stop codon in an mRNA sequence. RF2 has been Codon optimized for use in Escherichia coli and contains a N-Terminal histidine tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 587
    Illegal BglII site found at 1044
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None