Coding

Part:BBa_K2443051

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-10)


Leu RS

Leucine tRNA synthetase responsible for attaching leucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal histidine tag with a serine glycine linker.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 294
    Illegal NgoMIV site found at 1485
    Illegal AgeI site found at 955
    Illegal AgeI site found at 982
    Illegal AgeI site found at 1036
    Illegal AgeI site found at 1113
    Illegal AgeI site found at 1885
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None