Coding

Part:BBa_K2443048

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-10)


Gly RS beta

glutamic acid tRNA synthetase responsible for attaching glutamic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1698
    Illegal SapI site found at 55


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Categories
Parameters
None