Composite

Part:BBa_K2443000

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-04)


AlaRS optimized for expression in E. coli

Alanine tRNA synthetase responsible for attaching alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BglII site found at 720
    Illegal BamHI site found at 686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1124
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2524
    Illegal BsaI site found at 2701
    Illegal BsaI.rc site found at 1073
    Illegal SapI site found at 1808


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
protein