Part:BBa_K243027

His-FOS

This part is composed of a polyhistidine-tagged leucine zipper domain derived from the mammalian c-Fos protein. It can form DNA-binding homo- and heterodimers and can be used to target fused protein parts to specific DNA regions.

[http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS Heterodimeric Fos complex]

Usage and Biology

Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers and binds DNA as a dimer. Two classes of core DNA sequences, the sequences TRE (TGACTCA) and CRE (TGACGTCA), can be recognized by AP-1. Nine homologues of the AP-1 leucine zipper region have been characterized. All of them are able to form heterodimers, some also form homodimers. One of them is the so called c-Fos. Via their leucin zipper domains they interact with each other and bind DNA with their bipartite domain (Abate et al., Mol Cell Biol., 1991). The sequence for the bZip domains of c-Fos is linked to a His tag which allows protein purification using a Ni-NTA column. Soluble expression in Escherichia coli is possible.
In the research dimerized c-fos is normally used as a DNA binding molecule.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 19
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]