Composite

Part:BBa_K243014

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)

His-FluA-Middle Linker-Fok_i

This combination uses the benefits of an His-tag for purification. It is also linked with a FluroescineA-tag. The Middle Linker (GlySerGlyGly)x2 connects the parts and adds additional space between them to guarantee the independent function of FluA tag and the protein domain Fok_i.

Usage and Biology

This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243017 to built to a functional heterodimer. The FluA tag leads the part to DNA which is hybridized with an Fluorescein labeled oligonucleotide. The middle linker makes a distance from 24bp between the FluA and the linked Fok_i protein domain. The His-tag serves as purification tag for a Ni-NTA column purification.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/a His-FluA-Middle Linker-Fok_i