Part:BBa_K243014
His-FluA-Middle Linker-Fok_i
This combination uses the benefits of an His-tag for purification. It is also linked with a FluroescineA-tag. The Middle Linker (GlySerGlyGly)x2 connects the parts and adds additional space between them to guarantee the independent function of FluA tag and the protein domain Fok_i.
Usage and Biology
This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243017 to built to a functional heterodimer. The FluA tag leads the part to DNA which is hybridized with an Fluorescein labeled oligonucleotide. The middle linker makes a distance from 24bp between the FluA and the linked Fok_i protein domain. The His-tag serves as purification tag for a Ni-NTA column purification.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 272
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
n/a | His-FluA-Middle Linker-Fok_i |