Composite

Part:BBa_K242901

Designed by: Minerva Trejo, Laura Gomez, Libertad Pantoja, Abraham Avelar, Uriel Urquiza, Fernando Montaño, Arturo Velarde, Eduardo Soto, Adrian Granados, Willebaldo García, Enrique Quintana, Carlos Vargas, Enriqu   Group: iGEM09_LCG-UNAM-Mexico   (2009-10-21)


(pT3/T7-GFP-E3-E9-LuxI-T)+(pTet-LuxR-T)+(plux-RFP)+(plux-antisenseT3/T7-T)

This is a device that should confer protection to an E.Coli population against T3 and T7 bacteriophage infection. The system starts detecting RNApol of T3 or T7 by specific promoters. Transcription of toxins and production of AHL starts with the aim of kill the cell before more phages production. The surrounding cells receive the quorum molecule (AHL), binding with luxR result in activation of plux->transcription of RFP, just as a reporter. Also you start transcription of an antisense against T3 and T7 replication machinery. The last part has the intention to cause a delay in the production cycle of those phages so the device increase its efficiency

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3018
    Illegal AgeI site found at 3130
    Illegal AgeI site found at 4894
    Illegal AgeI site found at 5006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 726
    Illegal BsaI.rc site found at 5188


[edit]
Categories
Parameters
None