Part:BBa_K242900

[p(T3/T7)-GFP-(E3 + E9 toxins)]

This is a device for killing the cell if phage T3 or T7 is inside the cell.

RNA pol of T3 and T7 recognize the promoter and transcribe the toxic domain of colicin E3 and E9.

Also you transcribe LuxI, an AHL productor.

We expect high level of transcription with this promoter and polymerase.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2294
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 726