Composite
Part:BBa_K2423004
Designed by: iGEM Uppsala 2017 Group: iGEM17_Uppsala (2017-08-18)
Zeaxanthin to crocin enzymatic pathway
This part contains coding devices for CaCCD2 (BBa_K2423000), CsADH-2946 (BBa_K2423001), UGTCs2 (BBa_K2423002 and RFP(BBa_J4450). It these should catalyse the synthesis of α-crocin from zeaxanthin. The intended use was in con junction with BBa_K323122, which has been shown to produce zeaxanthin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1891
Illegal NheI site found at 1914
Illegal NheI site found at 3613
Illegal NheI site found at 3636 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2079
Illegal XhoI site found at 4199 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2486
Illegal AgeI site found at 1774
Illegal AgeI site found at 4372
Illegal AgeI site found at 4727
Illegal AgeI site found at 5878
Illegal AgeI site found at 5990 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3003
Illegal BsaI.rc site found at 672
Illegal SapI.rc site found at 2111
[edit]
Categories
Parameters
None |