Composite

Part:BBa_K2423003

Designed by: iGEM Uppsala 2017   Group: iGEM17_Uppsala   (2017-08-18)


Zeaxanthin to crocetin enzymatic pathway

This part is a fusion of BBa_2423000 and BBa_2423001. It contains the genes for the enzymes CaCCD2 and CsADH-2946. These should catalyse the synthesis of crocetin from zeaxanthin. The intended use was in conjunction with BBa_K323122, which has been shown to produce zeaxanthin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1891
    Illegal NheI site found at 1914
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2079
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2486
    Illegal AgeI site found at 1774
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3003
    Illegal BsaI.rc site found at 672
    Illegal SapI.rc site found at 2111


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Categories
Parameters
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