Composite

Part:BBa_K242151

Designed by: Enrique Paz   Group: iGEM09_LCG-UNAM-Mexico   (2009-10-20)


lacI promoter + lux I + TER + ptet-luxR-TER + lux pR - RFP


Quorum sensing device under lacI promoter. The sistem will induce RFP transcription under IPTG.

Be carefull about the inhibitors of lacI promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 863
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2658
    Illegal AgeI site found at 2770
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2019


[edit]
Categories
Parameters
None