Composite

Part:BBa_K242150

Designed by: Enrique Paz   Group: iGEM09_LCG-UNAM-Mexico   (2009-10-20)


(no promoter - AHL) + (ptet - luxR) + (quorum sensing inducible RFP)

The idea for this part is the chance to ligate any promoter before the AHL making enzyme to activate the quorum sensing device. Then we have constitutive transcription of LuxR and RFP activated by AHL-LuxR as a reporter.

This way all the quorum sensing systems could be activated by a desired promoter and for the response/sensing you only need a promoter dependant on AHL-LuxR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2450
    Illegal AgeI site found at 2562
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1811


[edit]
Categories
Parameters
n/a(no promoter - AHL) + (ptet - luxR) + (quorum sensing inducible RFP)