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Part:BBa_K242051

Designed by: Enrique Paz   Group: iGEM09_LCG-UNAM-Mexico   (2009-10-20)


Essential region of P4 bacteriophage genome


Bacteriophage P4 genome without the non-essential region nor the integrase gene. The use of this part is similar to a plasmid. You can clone standard parts in this viral vector. You can use this vector with a system for P4 production to encapsidate your constructions and transduce them.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2636
    Illegal NotI site found at 7087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 158
    Illegal BamHI site found at 171
    Illegal BamHI site found at 6566
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 343
    Illegal AgeI site found at 1378
    Illegal AgeI site found at 2200
    Illegal AgeI site found at 2494
    Illegal AgeI site found at 3433
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3512
    Illegal BsaI.rc site found at 5590


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