Composite

Part:BBa_K2418007

Designed by: Akos Harangozo and Akos Sukosd   Group: iGEM17_Szeged_SA_RMG   (2017-10-27)


Fully functional transcription unit for Methylococcus capsulatus

This is a fully functional transcription unit for Methylococcus capsulatus. It contains the a promoter, an extendend T7 promoter, an extended ribosome binding site, the gene of the lactate-dehydrogenase (LDH) and an extended T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 9
    Illegal BglII site found at 318
    Illegal BglII site found at 340
    Illegal BamHI site found at 1496
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 576


[edit]
Categories
Parameters
None