Plasmid

Part:BBa_K2418003

Designed by: Akos Sukosd   Group: iGEM17_Szeged_SA_RMG   (2017-10-26)


The full sequence of the modular broad-host-range expression vector pMHE7

This is the modular broad-host-range vector pMHE7. This vector is a synthetically made plasmid designed especially for Methylococcus capsulatus.

This part was not developed by the Szeged_SA_RMG team. For further information, please see Fodor, B. D. et al.: Modular Broad-Host-Range Expression Vectors for Single-Protein and Protein Complex Purification, Applied and Environmental Microbiology, 70th issue, 712–721 (2004)

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC348848/

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 175
    Illegal BamHI site found at 408
    Illegal XhoI site found at 312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 143
    Illegal NgoMIV site found at 1008
    Illegal NgoMIV site found at 4403
    Illegal AgeI site found at 848
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5428
    Illegal BsaI.rc site found at 3602


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