Part:BBa_K2417012

TEV Protease Cleavage Sequence

Protein sequence required for cleavage with TEV protease. Can be used as a linker between purification tags and proteins of interest, to allow specific cleavage of purification tag after use. TEV protease is sourced from the Tobacco Etch Virus and targets the recognition site ENLYFQ↓G, which is the amino acid sequence this DNA sequence encodes. Because the cleavage site is very sequence specific, it is a very useful protease to use on recombinant proteins because it will not indiscriminately digest the protein of interest, and will only cut at the site indicated.

References (1) Waugh, D.S. 2011, "An overview of enzymatic reagents for the removal of affinity tags", Protein Expression and Purification, vol. 80, no. 2, pp. 283-293. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]