Part:BBa_K2417008
6x Poly-histidine Tag codon optimised for use in E. coli
We have codon-optimised the DNA-sequence encoding 6 adjacent histidine residues to be expressed in E. coli efficiently. A poly-His tag is utilized to bind it to immobilized ions on a chromatography affinity column (often nickel, cobalt or copper), so as to isolate from a mixed solution the tagged protein of interest. The tagged protein will bind to the immobilized ions at a much stronger affinity than other proteins, so that all except the protein of interest can be eluted from the column with a low salt buffer. The protein of interest is then eluted with a high salt buffer.
The His-tag is useful as it is small (so can be easily added to either the N or C terminus) and a widely used method of purification, so its use can be validated by many previous experiments.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |