Translational_Unit

Part:BBa_K2415004

Designed by: Yunping Lin   Group: iGEM17_GZHS-United   (2017-10-21)


Cry4Ba-eGFP

This part is consititued by Cry4Ba(BBa_K2415000) coding sequence and eGFP(BBa_K404316).It is designed for the test the expression of Cry4Ba.A linker peptide encodes Trp-Trp-Trp-Ser is in the middle of the sequence to connect the two proteins without influencing activity of the two proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 69
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 475
    Illegal BsaI.rc site found at 4061


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Categories
Parameters
None