Composite

Part:BBa_K2414003

Designed by: Takashi Hirao   Group: iGEM17_HokkaidoU_Japan   (2017-10-27)


phytase with cysteine module

This part is composed of phytase with self-assembling region and cysteine module under lac promoter.

HokkaidoU_Japan 2017 analyzed this part. As temperature rises, this phytase's activity falls as below graph. Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer Digest phytic acid in above situation with sample phytase solution 10μl, 37degrees celcius, 10 min. After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes.

T--HokkaidoU_Japan--K2414003.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1585
    Illegal BglII site found at 1639
    Illegal BamHI site found at 352
    Illegal BamHI site found at 1543
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 418
    Illegal NgoMIV site found at 819
    Illegal AgeI site found at 1502
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1311


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Categories
Parameters
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