phytase with cysteine module
This part is composed of phytase with self-assembling region and cysteine module under lac promoter.
HokkaidoU_Japan 2017 analyzed this part. As temperature rises, this phytase's activity falls as below graph. Protocol: 50μl 7.5 mM Phytic acid 190μl CH3COOH Buffer Digest phytic acid in above situation with sample phytase solution 10μl, 37degrees celcius, 10 min. After digestion, using AMM solution (2ml of 10mM NH4Mo7O2.4H2O: 5N H2SO4: Acetone= 1:1:2) analyze Optical Absorbance. The more Pi are there, the more yellow AMM becomes.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 1585
Illegal BglII site found at 1639
Illegal BamHI site found at 352
Illegal BamHI site found at 1543
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 418
Illegal NgoMIV site found at 819
Illegal AgeI site found at 1502
- 1000Illegal SapI.rc site found at 1311