Composite

Part:BBa_K2412003

Designed by: Jennifer Fang   Group: iGEM17_ColumbiaNYC   (2017-10-27)


circuit for shRNA knockdown of EGFR

This is a IPTG-inducible circuit with a T7 promoter that produces an shRNA that knocks down EGFR from cancer cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 43
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 89


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Categories
Parameters
None