Composite

Part:BBa_K241000

Designed by: John Heil   Group: iGEM09_Waterloo   (2009-10-20)


Nuclease construct, no sense promoter.

To tightly repress the nuclease genes (T7 gene 6, PmeI) an antisense promoter and the cI repressor are used.

The promoter for expression of the integrase plasmid (the sense promoter) is not on this part. However, the design calls for the Lambda Pr promoter.

The part is designed to selectively degrade DNA in vivo.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1497
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1599
    Illegal BsaI.rc site found at 223
    Illegal BsaI.rc site found at 912
    Illegal BsaI.rc site found at 1292


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