Part:BBa_K2407112

CdMT: express a metallothionein to combine with cadmium ions in yeast

Metallothioneins (MTs) form a class of cysteine-rich polypeptides that are capable of binding a large number of metal ions. LlMT is a kind of metallothioneins from the snail Littorina littorea and is capable of binding 9 Zn2+ or 9 Cd2+ ions. we obtained its gene from Gene synthesis company after codon optimization.

Characterization

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As an essential part in our heavy metal project, LIMT gene is expressed in our yeast after switching. To characterize the effect of LIMT gene , strains of S. cerevisiae Syn V containing this gene is accumulated in YPD Medium without cadmuim ions.Then,cadmuim ions is added into the culture medium so that the concentration of cadmuim ions in the environment is 8mg/L. Atomic absorption spectroscopy is used to measure the concentration of cadmuim ions in the supernatant at equal intervals. The results are plotted as the adsorption curve in the figure.

Fig 1. the concentration curve of cadmuim ions

Figure clearly shows the change of the concentration of cadmuim ions in the supernatant. The concentration of cadmuim ions declines over time and S.C-Cd(genetically-engineered yeast) is more capable of binding cadmium ions than control group(BY4741).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]