Part:BBa_K2407005

Cre Recombinase

This part is a sequence coding the Cre recombinase. Cre recombinase is a tyrosine recombinase enzyme originally derived from P1 bacteriophage. Cre recombinase can specifically recognize a pair of Loxp sites which consists of two 13 bp palindromic sequences flanking an 8bp spacer region, and catalyzes the recombination event between the sites. The result of this recombination event is dependent on the relative orientation of the Loxp sites.

Reference:Controlled expression of Cre-EBD may then lead to stochastic rearrangements of chromosome segments flanked by loxPsym sites, with deletions and inversions in principle equally likely based on the relative orientation of the loxPsym sites in the recombination junction.Shen, Y., Stracquadanio, G., Wang, Y., Yang, K., Mitchell, L. A., & Xue, Y., et al. (2016). Scramble generates designed combinatorial stochastic diversity in synthetic chromosomes. Genome Research, 26(1), 36.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 316
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2
  
• 1000
  COMPATIBLE WITH RFC[1000]

d