Protein_Domain
FLO1

Part:BBa_K2407004

Designed by: Xinyue Cao   Group: iGEM17_Tianjin   (2017-10-12)


FLO1 gene triggers the yeast flocculation

S.cerevisiae flocculation FLO1 genes codes the FLO1 protein and is located in Saccharomyces cerevisiae strain S288c chromosome I from 203403bp to 208016bp. FLO1 protein spans the cell membrane, which C terminal has a GPI anchored protein and N terminal is in extracellular space. FLO1 protein's N terminal can combine mannose on adjacent cell's cell wall so as to trigger the accumulation of yeasts.

Reference: FLO gene's function contains Lectin-like protein involved in flocculation; cell wall protein that binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; important for co-flocculation with other yeasts, mediating interaction with specific species.Kobayashi, O., Hayashi, N., Kuroki, R., & Sone, H. (1998). Region of Flo1 proteins responsible for sugar recognition. Journal of bacteriology, 180(24), 6503-6510.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 55
    Illegal SpeI site found at 2296
    Illegal SpeI site found at 2701
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 952
    Illegal SpeI site found at 55
    Illegal SpeI site found at 2296
    Illegal SpeI site found at 2701
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4336
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 55
    Illegal SpeI site found at 2296
    Illegal SpeI site found at 2701
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 55
    Illegal SpeI site found at 2296
    Illegal SpeI site found at 2701
    Illegal AgeI site found at 850
    Illegal AgeI site found at 892
    Illegal AgeI site found at 1162
    Illegal AgeI site found at 1297
    Illegal AgeI site found at 1432
    Illegal AgeI site found at 1567
    Illegal AgeI site found at 1702
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1189
    Illegal BsaI.rc site found at 1324
    Illegal BsaI.rc site found at 1459
    Illegal BsaI.rc site found at 1594
    Illegal BsaI.rc site found at 2134


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