Protein_Domain

Part:BBa_K2407003

Designed by: Yushuang Wang   Group: iGEM17_Tianjin   (2017-10-12)


CYC1 terminator

CYC1 terminator is a DNA fragment with a special structure, a marker for end of transcription which is located in Saccharomyces cerevisiae strain S288c chromosome X from 526690bp to 526939bp .It is necessary for a vector to have this three parts, promoter, interest gene and terminator.

Reference: CYC1 terminator is commonly used in Saccharomyces cerevisiae .Curran, K. A., Morse, N. J., Markham, K. A., Wagman, A. M., Gupta, A., & Alper, H. S. (2015). Short synthetic terminators for improved heterologous gene expression in yeast. Acs Synthetic Biology, 4(7), 824-832.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None