Part:BBa_K2404013

Luc+ gene under control of the 35S CaMV promoter

We generated a luciferase construct to test the efficacy of the tobacco leave expression system.

This construct contains the 35S-OTMV promotor the luciferase coding sequence and the Nos terminator.

This construct was introduced into agrobacteria strain GV3101, infiltrated into leaves of Nicotiana benthamiana and the luciferase expression was assessed following addition of luciferin. This full experimental details and results are outlined in the Design and Experience pages.

This part is contained within the Phytobrick level 1 pGB-A1 plasmid.

Our luc+ reporter gene under control of the 35S constitutive promoter from the cauliflower mosaic virus. Luciferase reporter assays were carried out using this construct, with the aim to compare this reading to that of other constructs. This part has the NosT terminator from the Nopaline synthase gene, native to Agrobacterium Tumefaciens . This part should be used as a reporter gene to test 35S expression, relative to another promoter/gene construct.

Sequence and Features
Sequence detail:
1-6: BsmBI
9-1439: 35S-OTMV
1440-3104: LUC+
3105-3371: NosT
3381-3386 :BsmBI

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 13
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 13
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 13
  Illegal BglII site found at 400
  Illegal BglII site found at 1146
  Illegal BglII site found at 2270
  Illegal BamHI site found at 2116
  Illegal XhoI site found at 1350
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 13
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 13
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1442