Part:BBa_K2404001
A Thyroid Stimulating Hormone antagonist with His-Tags
This is a coding sequence of a gene that produces a protein that competitively inhibits thyroid stimulating hormone (TSH). This includes a 6xHis-tags to aid protein purification.
TSH was first generated and characterised by [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558]. The protein includes two parts, the beta subunit from Thyrotropin (TSH) fused to a common alpha subunit shared by hormone glycoproteins. This is linked by a short CTD linker sequence. This TSH antagonist has been modified to remove glycosylation sites that exist on both subunits. As a result this antagonist binds to the TSH receptor but does not activate it.
TSH has been expressed in a murine expression system and shown to inhibit the auto-immune antibodies that cause in Graves' disease ([http://www.pnas.org/content/113/5/1244.full Saxena et al (2015). PNAS 113, 1244-129]).
We intend to use this TSH protein as a therapeutic treatment to treats hyperthyroidism in humans.
This part has been cloned ito pSB1C3 using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard. It is flanked by BsaI sites together 5' aatg and 3' gctt cloning sequences for subsequent cloning into a level 1 plasmid.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 540
Illegal PstI site found at 582 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 540
Illegal PstI site found at 582 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 795
Illegal XhoI site found at 607 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 540
Illegal PstI site found at 582 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 540
Illegal PstI site found at 582 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 827
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