Part:BBa_K2401013

Tired

Thiazolinyl imide reductase, this protein is a common but unique catalysator in NRS for peptides including cyclized cysteine, which this reductase forms out of linear cysteine creatin a thiazolin ring.

To do so cysteine needs to be bound to a a C-donator by its amino group. Without a C-Atom to connect cysteine cannot cyclize.

Which explains why a low peak was observable while expressing it in cells were structures, as intermediates, similar to our target siderophores (Pyochelin, Yersiniabactin) might be formed. While we could not detect a shift in fluorescence in cell debris with tired in dissolved in the media.

Since the measurement data is still quite inconclusive this needs further investigation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]